Calcium-binding protein of chorioallantoic membrane: identification and development expression.

Calcium-binding protein of the chick chorioallantoic membrane. II. Vitamin K-dependent expression

A simple method was devised for the maintenance of the chorioallantoic membrane (CAM) of chick embryos in organ culture. Explants of CAM survived for up to 5 days in this system and retained the characteristic three-layered morphology (ectoderm, mesoderm, and endoderm). Induction of the CAM calcium-binding protein (CaBP) by effectors of calcium metabolism was studied in these organ cultures. Vi...

Identification and characterization of a calcium-binding protein in the mouse chorioallantoic placenta.

Mouse chorioallantoic placenta contains a specific calcium-binding protein (MCaBP). A procedure involving gel filtration and ion-exchange chromatography was developed to purify the MCaBP. The MCaBP activity increased as a function of embryonic gestation and was highly specific for Ca2+. The MCaBP is a monomeric protein of Mr 57000, with pI 4.7. Specific antibodies were prepared against the MCaB...

Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy

Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization

The preparation of a specific antiserum (anti-CaBP) against the calcium-binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding w...

expression and distribution of calcium-binding protein s100p in human placenta during pregnancy

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